B7157
Sigma
Baculovirus Expression Vectors: A Laboratory Manual
| ISBN-10: | 0-19-509131-0 |
| ISBN-13: | 978-0-19-509131-1 |
Description
| General description | This laboratory manual simplifies the selection of the most appropriate vector design for a given problem and describes each step in the implementation process from vector construction to large-scale protein production. |
Properties
| publication info | D.R. O'Reilly, et al., Oxford University Press, US, 1994, 347 pp., soft cover |
Table Of Contents
| Table of Contents | PART I: AN OVERVIEW OF BACULOVIRUSES 1. Virus Structure and the Infection Process 2. Gene Organization, Regulation, and Function 3. Virus-Host Interactions 4. Summary of Baculovirus Features Relevant to Expression Factors PART II: CHOOSING A TRANSFER PLASMID AND PARENT VIRUS 5. Choice of Virus and Host Species 6. Choice of Transfer Plasmid 7. Available Transfer Plasmids 8. Choosing a Parent Virus for Use in Vector Construction 9. Optimizing Expression: Tailoring the Heterologous Gene to the Transfer Plasmid and the Baculovirus Expression System PART III: METHODS FOR VECTOR CONSTRUCTION AND GENE EXPRESSION 10. Safety Considerations 11. Insect Cell Culture 12. Virus Methods 13. Contransfection and Recombinant Virus Identification 14. Characterizing Recombinant Gene Expression 15. Post-Translational Modification PART IV: METHODS FOR SCALE-UP OF PROTEIN PRODUCTION AND USE OF INSECT LARVAE 16. Scale-Up of Protein Production in a Stirred Bioreactor, David L. Clemm 17. Scale-Up of Protein Production in an Airlift Fermenter, Cheryl Isaac Murphy 18. Insect Rearing and Infection Appendix 1: Nucleotide Sequence of AcMNPV Polyhedrin Region Appendix 2: Nucleotide Sequence of AcMNPV p10 Region Appendix 3: List of Genes Expressed in the Baculovirus Expression System Appendix 4: Suppliers Appendix 5: Stock Solutions Appendix 6: Excel Spreadsheet for TCID50 Calculation References Index |
